In vitro multiplication of banana (Musa sp.) cv. Grand Naine

Abstract

A micropropagation method is described for banana ( Musa Spp .) Cv. Grand Naine. Suckers were surface sterilized with HgCl 2 (0.1%) for 6 min which gave minimum contamination with maximum culture establishment. Of various treatment combinations, Murashige and Skoog (MS) medium + BAP 4.00 mg/l with IAA 2.00 mg/l resulted in maximum establishment of cultures in lesser time. MS medium + BAP 4.00 mg/l + IAA 2.00 mg/l gave maximum multiple shoots. Maximum rooting was obtained on MS medium (half strength) supplemented with IBA 1.00 mg/l and activated charcoal 200 mg/l. Keywords: Grand naine, micro propagation, murashige and skoog medium, Musa spp. and suckers. African Journal of Biotechnology , Vol 13(27) 2696-2703