ANTIBACTERIA ACTIVITY OF GASTROPOD ASSOCIATION BACTERIA FROM MANGROVE ECOSYSTEM AGAINST BACILLUS CEREUS AND ESCHERICHIA COLI AND IT’S POTENCY OF APPLICATION FOR BELANAK FISH (MUGIL SUBVIRIDIS)

Abstract

Gastropod association bacteria from mangrove ecosystem have a great potency as antibacterial. The contamination of Escherichia coliand Bacillus cereuscause damage in fish product. The aims of thisresearch areto identifygastropod association bacteria that can inhibit E. coliand B. cereusgrowth, as well as the contamination bacteria in Belanak (Mugil subviridis).Gastropods sample Certhideopsilla alata, Cerithidea quoyii, Cassidula aurisfelis, Cassidula nucleus, Telescopium telescopiumwere collected at Mangrove Education Park, Tugu, Semarang. The research procedure wasisolation, antibacteria test, biochemicalidentification, and the potency to inhibit contaminant bacteria in Belanak fish (Mugil subviridis).There were 61 bacteria isolated. Thirteen isolates were able to inhibit E. coliand eight to B. cereus. Supernatant of GMT 3.2.5 and GMT 4.1.3 have the most widely inhibition zone, (8,48 mm and 7,84 mm). Biochemistry identification shown that GMT 3.2.5 and GMT 4.1.3 has identic characteristic with genus Micrococcus sp. and Bacillus sp. Total plate count (cfu/ml) of bacteria from Belanak fish flesh that soaked by Micrococcus sp. GMT 3.2.5 and Bacillus sp. GMT 4.1.3 supernatant were 115 x 104and 265 x 103colony. The colony were less than negative control (2312 x 103colony). The association bacteriacaninhibit contaminant bacteria in Belanak fish.