Taurine enhances mouse cochlear neural stem cells proliferation and differentiation to sprial gangli through activating sonic hedgehog signaling pathway

Abstract

To investigate the molecular mechanism underlying taurine-stimulated proliferation and differentiation of cochlear neural stem cells (NSCs) and potential involvement of Sonic Hedgehog (Shh) pathway. The NSCs were characterized with immunofluorescence stained with nestin antibody. Cell viability was determined by MTT assay. The relative proliferation was measured by BrdU incorporation assay. The morphologic index was measured under light microscope. The relative protein level was determined by immunoblotting. Here we presented our findings that taurine stimulated proliferation and neurite outgrowth of NSCs, which was completely abolished by Shh inhibitor cyclopamine. In addition, cyclopamine antagonized taurine’s effect on glutamatergic and GABAergic neuron population via suppressing expressions of Ptc-1, Smo and Gli-1. Our data supported the critical role of Shh pathway underlying the protective effect of taurine on auditory neural system.