A simple, sensitive determination of ganciclovir in infant plasma by high-performance liquid chromatography with fluorescence detection

Abstract

This study developed a simple and sensitive method using reversed-phase high-performance liquid chromatography (HPLC) for ganciclovir (GCV) plasma concentrations in cytomegalovirus infectious infants with hearing loss. The method involves a simple protein precipitation procedure that uses no solid-phase or liquid-liquid extraction. The HPLC separation was carried out on a Cadenza CD-C18 column (3 μm, 4.6 mm × 150 mm) with phosphate buffer (pH 2.5, 25 mM) containing 1% methanol-acetonitrile mixture (4:3, v/v) as a mobile phase at a 0.7 mL/min flow rate. GCV was detected using a fluorescence detection (λex/em: 265/380 nm). The quantification limit was 0.025 μg/mL for 100 μL of plasma sample at which good intra-and inter-assay coefficient of variation values (< 4.96%) and recoveries (94.9-96.5%) were established.