Genes transactivated by hepatitis C virus core protein, a microarray assay

Abstract

Aim: To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection. Methods: Reverse transcribed cDNA was subjected to microarray assay. The coding gene transactivated by HCV core protein was cloned and analyzed with bioinformatics methods. Results: The expressive vector of pcDNA3.1(-)-core was constructed and confirmed by restriction enzyme digestion and DNA sequencing and approved correct. mRNA was purified from HepG2 and HepG2 cells transfected with pcDNA3.1(-)-core, respectively. The cDNA derived was subjected to microarray assay. A new gene named HCTP4 was cloned with molecular biological method in combination with bioinformatics method. Conclusion: HCV core is a potential transactivator. Microarray is an efficient and convenient method for analysis of differentially expressed genes. © 2005 The WJG Press and Elsevier Inc. All rights reserved.