Serotyping and analysis of produced pigments kinds by Pseudomonas aeruginosa clinical isolates

Abstract

Background/Aim. Pseudomonas aeruginosa (P. aeruginosa) is devided into 20 serotypes on the base of the International Antigenic Typing Scheme. P. aeruginosa serotyping is important because of few reasons but epidemiological is the most important. The aim of the study was serotyping of P. aeruginosa clinical isolates, analysing of single clinical isolates P. aeruginosa present in the particular samples, and analysing of pyocianin and fluorescin production in different isolates of P. aeruginosa. Methods. A total of 223 isolates of P. aeruginosa, isolated in the microbiological laboratory of the Health Center ?Aleksinac?, Aleksinac, were examinated. P. aeruginosa isolates were put on the pseudomonas isolation agar, pseudomonas agar base, acetamid agar, asparagin prolin broth, pseudomonas asparagin broth, Bushnnell-Haas agar, cetrimid agar base, King A and King B plates, plates for pyocianin production, plates for fluorescin production and tripticasa soya agar (Himedia). Polyvalent and monovalent serums were used in the agglutination (Biorad). Pigment production was analysed on the bases of growth on the plates for pyocianin and fluorescin production. Results. Serologically, we identificated the serovars as follows: O1, O3, O4, O5, O6, O7, O8, O10, O11 and O12. O1 (38%) was the most often serovar, then O11 (19%) and O6 (8.6%). A total of 18.6% (42) isolates did not agglutinate with any serum, whereas 21 isolates agglutinated only with polyvalent serum. The majority of P. aeruginosa isolates produced fluorescin, 129 (58.54%), 53 (22.94%) produced pyocianin whereas 49 (21.21%) isolates produced both pigments. Conclusion. P. aeruginosa was isolated most of the from urine, sputum and other materials. The majority often serovars were O1, O6 and O11. The most of isolates produced fluorescin (58.54%), while 22.94% producted pyocianin and 21.21% both pigments.Uvod/Cilj. Prema International Antigenic Typing Scheme Pseudomonas aeruginosa (P. aeruginosa) deli se na 20 serotipova. P. aeruginosa jedan je od najvaznijih uzrocnika bolnickih infekcija, pa je njegova serotipizacija vazna iz epidemioloskih razloga. Cilj ove studije bio je serotipizacija klinickih izolata P. aeruginosa, analiza prisustva pojedinih serotipova P. aeruginosa u pojedinim bolesnickim materijalima i analiza proizvodnje fluorescina i piocijanina kod razlicitih serotipova. Metode. U mikrobioloskoj laboratoriji Zdravstvenog centra ?Aleksinac? ispitana su 233 izolata P. aeruginosa. Izolati P. aeruginosa presejavani su na: pseudomonas izolacioni agar, pseudomonas agar bazu, acetamidni agar, asparagin prolin bujon, pseudomonas asparagin bujon, Bushnnell-Haasov agar, cetrimid agar bazu, King A i King B podlogu, podlogu za proizvodnju piocijanina, podlogu za proizvodnju fluorescina i triptikaza soja agar (Himedia). Za aglutinaciju korisceni su polivalentni i monovalentni serumi (Biorad). Proizvodnja pigmenata pracena je na osnovu porasta na podlogama za proizvodnju fluorescina i piocijanina. Rezultati. Seroloski, identifikovani su sledeci serotipovi: O1, O3, O4, O5, O6, O7, O8, O10, O11 i O12. Najprisutniji bili su serotipovi O1 (38%), O11 (19%) i O6 (8,6%). Izolati koji nisu pokazali pozitivnu reakciju aglutinacije ni sa jednim serumom cinili su 18,6% (42) uzoraka, dok je 21 izolat aglutinisao samo sa polivalentnim serumima. Najveci broj izolata P. aeruginosa proizvodio je fluorescin (58,54%), 53 (22,94%) piocijanin, a 49 (21,21%) sojeva oba pigmenta. Zakljucak. P. aeruginosa izolovan je najcesce iz urina, sputuma i drugih bolesnickih materijala. Najcesce izolovani serotipovi P. aeruginosa bili su O1, O6 i O11. Najveci broj izolata P. aeruginosae proizvodio je fluorescin (58,54%) dok je 22,94% proizvodio piocijanin, a 21,21% oba pigmenta.