Channeled ECM-based nanofibrous hydrogel for engineering vascularized cardiac tissues

Abstract

Hydrogels are widely used materials for cardiac tissue engineering. However, once the cells are encapsulated within hydrogels, mass transfer to the core of the engineered tissue is limited, and cell viability is compromised. Here, we report on the development of a channeled ECM-based nanofibrous hydrogel for engineering vascularized cardiac tissues. An omentum hydrogel was mixed with cardiac cells, patterned to create channels and closed, and then seeded with endothelial cells to form open cellular lumens. A mathematical model was used to evaluate the necessity of the channels for maintaining cell viability and the true potential of the vascularized hydrogel to form a viable cardiac patch was studied.