Simultaneous Quantitation of Organic Acids and Monosaccharides by High-Performance Liquid Chromatography

Abstract

Objective: a fast, simply, reliably and accurately high-performance liquid chromatography (HPLC) method for the simultaneous quantitation of commonly used organic acid and monosaccharides in food industry such as benzoic, malic, citric and quinic acid, fructose, glucose has been developed. Methods: In order to obtain a satisfactory separation in a short elution time, various HPLC operational factors like composition of mobile phase, column temperature and flow rate affecting analysis had been optimized Results: The optimum determination conditions are as follows: for a CAPCELL PAK NH2 column, flow rate of 1 mLimin, 75% acetonitrile (pH 2.25 adjusted by phosphoric acid) as mobile phase, column temperature of 35 C. Under these conditions, this method could achieve adequate separation within 18 mm and show the excellent linearity (R-2 0.999), high precision (R.S.D 5%) and good accuracy (The recovery closed to 100%). Moreover, the procedure of this method is very sample, involves little sample preparation which just consists of dilution and filtration before injection, and the cost of this method is relative low compared with ion-exchange HPLC method. Conclusion: it is promising method to simultaneous quantitation of organic acids and monosaccharides in food industry.