Abstract
A purified endoglucanase (Eg) from Bacillus subtilis and crude Eg preparations from Myrothecium verrucaria and Trichoderma reesei were denatured by heat in the presence of SDS and separated by using SDS polyacrylamide gel electrophoresis. Eg zymogram activity bands were detected directly in carboxymethylcellulose-laden separating gels after enzyme renaturation. Denaturing zymogram analysis was a simple method for the separation and detection of Egs. © 1994 Society for Industrial Microbiology.
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CITATION STYLE
Holt, S. M., & Hartman, P. A. (1994). A zymogram method to detect endoglucanases from Bacillus subtilis, Myrothecium verrucaria and Trichoderma reesei. Journal of Industrial Microbiology, 13(1), 2–4. https://doi.org/10.1007/BF01569654
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