Propagation of non-adherent HL-60 cells in batch cultures maintained in static and wave-type agitated systems

Abstract

Typiclly pplied sttic (i.e. non-gitted) cultures do not provide sucient conditions for ecient propgtion of suspended non-dherent cells, in generl. Fesibility of smll-scle wve-type gitted single-use biorectors for gentle gittion underlies pplicbility of such systems for scling-up of frgile biomss of niml cells. The bsic im of the study ws to compre the results of non-dherent HL-60 cell propgtion performed referentilly s the btch culture in typicl sttic (i.e. non-gitted) disposble culture sks (50 cm3 of culture medium) nd in ReadyToProcess WAVETM25 biorector system (GE Helthcre) equipped with disposble culture bg (300 cm3 of culture medium) subjected to continuous wve-type gittion. The density nd vibility of HL-60 cells were signicntly higher for the bioprocess subjected to wve-type gittion, thn in the reference sttic culture. The vlues of the specic rte of glucose consumption per cell (rglc/cell) exhibited by HL-60 cells mintined in the system with continuous wve-type gittion ws signicntly lower (i.e. up to more thn 42%) thn the vlues noted for the sttic culture, for exctly the sme time-points of two compred cultures. The results of the studies undoubtedly nd comprehensively conrmed the pplicbility of the studied disposble biorector with wve-induced gittion s the right pltform for proceeding the propgtion of non-dherent HL-60 cells nd for providing the culture conditions required by HL-60 cells for sustinble metbolism.