Separation of different types of monosaccharides by polyacrylamide column chromatography

Abstract

A high-throughput polyacrylamide gel chromatography was effectively tested to separate different types of monosaccharides. These occur for example in the complex bacterial LPS (lipopolysaccharide) structures as well as in other compounds. Here, a Bio-Gel P-2 column eluted only with water at ambient temperature afforded a convenient separation of the main structural types of neutral, charged, or highly polar hexoses, as well as N-acetylated hexose derivatives. These can be easily isolated on the semi-preparative scale by this method. The method was evaluated with a mixture of all basic types of monosaccharides, and repeatedly an efficient resolution was achieved. As an example of a complex structure, the monosaccharide constituents of the delipidated bacterial lipopolysaccharides (dLPS) of V. cholerae O135 were effectively separated after acid hydrolysis. © 2011 Verlag der Zeitschrift für Naturforschung.