GROWTH STIMULATION OF Lactococcus lactis sps BY XYLOOLIGOSACCHARIDES PRODUCED USING SINGLE FERMENTATIVE STEP FROM LIGNOCELLULOSIC WASTE BY Massilia Timonae B2YR

Abstract

As a source of xylan, lignocellulose waste such as wheat bran and aquatic weed Pistia stratiotes were employed, together with potassium nitrate as a nitrogen source, which were optimized using the Box-Behnken design to increase the production of xylooligosaccharides. The yield of xylooligosaccharides increased to 2.05-fold in the optimized medium than non-optimized medium. The produced xylooligosaccharides showed a peak at 890-900 cm-1 confirming the presence of β-glycosidic linkages when detected by Fourier Transform Infra-Red (FT IR). The prebiotic effect of produced xylooligosaccharides was studied on two strains named Lactococcus lactis Cl MN515342 and Lactococcus lactis Cp MN515343 isolated from curd samples. The growth of these Lactococcus strains was found to be stimulated. A log Colony Forming Unit/ml value of 7.813 and 8.146 for Cp and Cl was observed in a medium incorporated with XOS. The Short-Chain Fatty Acid (SCFA) production by Lactococcus lactis in a medium incorporated with xylooligosaccharides was detected by GCMS. The use of lignocellulosic waste for the production of xylooligosaccharides reduces the production cost. The xylooligosaccharides produced by Massilia timonae has shown promising result in stimulating the growth of Lactococcus lactis. Thus, XOS can be incorporated in curd or Yoghurt along with the Lactococcus lactis and used as synbiotic food.