Evaluation of EDTA as the chelator in the biuret reagent

Abstract

Use of ethylenediaminetetraacetate (EDTA) as the chelating agent in the biuret reaction (in place of tartrate or citrate) was investigated. This suggested modification of the reagent results in lower color yields and prolongs the time for color development. The EDTA reagent is more stable if color development is hastened by heating, but conditions are described under which the more customary reagent may be so used.