Antihyperglycemic activities ethanol extract of musa paradisiaca l stems and determination of its active components using gc-ms

Abstract

Various research on antihyperglycemic is continuously performed due to the fact every year the charge of diabetes patients increases. This study aims to find natural potentials that can be developed as an antihyperglycemic because existing synthetic drugs give unfavorable effects if used continuously. In this study, the natural potency of Musa Paradisiaca L was developed as an anti-hyperglycemic agent with the hope that its use would not cause a prolonged effect. The extract of ethanol’s Musa Paradisiaca L stem (EEMP) obtained by maceration was pretested by phytochemical screening. And obtained the presence of secondary metabolites, namely: Terpenoids, steroids, and saponins. Testing with GC-MS was also carried out to determine the active compound in ethanol extract which has potential as anti-hyperglycemia. The chromatogram results showed that the active compounds detected were: lupeol, stigmasterol, Cyclolanostan, and obtusifoliol. Previous studies of these components were able to lower blood sugar levels. The antihyperglycemic test was carried out in vivo on alloxan-induced mice. The treatment of diabetic mice was divided into 5 groups, namely: positive control, standard treatment, and treatment of extracts (100, 200, and 300 mg/kg BW) was given orally. The progression of decreasing effect of extract and glibenclamide at the 8th day limit after alloxan induction. The study showed that there were not have significant differences between treatment EECL and glibenclamide. The hyperglycemic test results clearly show that the ethanol extract of Musa paradisiaca L stem has the potential in antihyperglycemic treatment.