PCR-RFLP Genotyping of Point Mutations in Caenorhabditis elegans

Abstract

[Abstract] This protocol describes the basic principle of PCR/restriction digest genotyping of point mutations in worms, based on the principle of Restriction Fragment Length Polymorphism (RFLP) analysis. This type of genotyping is particularly useful when phenotypic analysis of animals carrying point mutations is difficult (e.g., in a complex genetic background).