Downregulation of the expression of GLUT1 plays a role in apoptosis induced by sodium butyrate in HT-29 cell line

Abstract

The regulation of glucose and sodium butyrate transporters(glucose transporter1-5 and Monocarboxylate transporter 1) and their relationship with cell apoptosis induced by sodium butyrate in colonic caner cell line HT-29 were studied. Cell apoptosis was detected by flow cytometric assay. The expression of MCT1 and GLUT1-5 mRNA were detected by RT-PCR and the uptake of glucose was detected using 2-deoxy-[ 3H]glucose. The expression of bax and bcl-x/l were detected by westernblot assay. We found that sodium butyrate induced apoptosis in HT-29 cell line. The expression of GLUT1 mRNA, bcl-x/l, as well the uptake of glucose was inhibited by sodium butyrate. The expression of MCT1 and GLUT2, GLUT3, GLUT5 was not regulated by sodium butyrate. However, the concentration of glucose had positive correlation with the expression of bcl-x/l protein and negative correlation with the apoptosis induced by sodium butyrate. All the results suggested that downregulation of the expression of GLUT1 was associated with the apoptosis induced by sodium butyrate in HT-29 cell line. © 2006 by MDPI.