Efeito do extrato aquoso de própolis marrom sobre a produção de ifn-γ após imunização contra parvovírus canino (CPV) e coronavírus canino (CCoV)

Abstract

The purpose of this study was to evaluate the imunnostimulatory adjuvant capacity of water extract from brown propolis (WEBP) when added to a vaccine against canine parvovirus (CPV) and canine coronavirus (CCoV), regarding the production of IFN-γ. Mice were vaccinated with CPV and CCoV (3.0 × 10 6 TCID 50) with or without 400 μg/dose of WEBP. Thirty days after the third dose, splenocytes were cultured to measure the expression levels of IFN-γ mRNA in the immunized animals. Increased levels of IFN-γ mRNA expression for CCoV were evidenced by RT-PCR, in the splenocytes of mice inoculated with the vaccine containing 400 μg/dose of WEBP, demonstrating the ability of propolis to stimulate cellular immune responses against the antigens of this virus. In contrast, the levels of IFN-γ to CPV were not influenced by the presence of WEBP.