Green Synthesis Using Klebsiella pneumoniae as well as its Execution onto Textiles for Microbe Resistance

Abstract

Microorganisms like bacteria and yeast and their use in the combination of nanoparticles is a basically a recent phenomenon. These microorganisms lessen the toxicity by decreasing the ions of metal or through the creation of complexes which are insoluble along with the metal's ions (as metal sulfides) as colloidal elements. The present study focuses on bio-synthesis of silver nanoparticles by the bacterium Klebsiella pneumoniae. The reduction of AgNO3 to Ag nanoparticles was due to the extracellular production of the enzyme nitrate reductase by the K. pneumoniae into the medium. The particles were characterized by SEM. The biosynthesized Ag nanoparticles were padded on the fabrics of cotton by cure method of dry pad. The preliminary antimicrobial activity was performed by disc diffusion method against Escherichia coli and Staphylococcus aureus. The antimicrobial property of the treated fabrics were confirmed qualitatively and quantitatively by parallel streak (AATCC- 147) and challenge test (AATCC -100) respectively. SEM results revealed the form of the nano particles were in range of 50 - 60 nm. An area of inhibition of 7 mm as well as 5 mm were found against S.aureus and E.coli respectively by disc diffusion test. Parallel streak method also confirmed the anjinicrobial activity. The results of the challenge test revealed the fabrics treated with biosynthesized nanoparticles depicted 100% minimum point in comparision with S.aureus and 99% reduction against E.coli.