Sequence analysis of the 5′-untranslated region of the human H1 histamine receptor-encoding gene

Abstract

A clone containing the H1 histamine receptor (H1HR)-CnCOdUIg gene was isolated from a human genomic DNA library. The 5′-UTR of the H1HR gene reported here differs upstream from bp -142 from that reported previously [Fukui et al., Biochem. Biophys. Res. Comm. 201 (1994) 894-901]. PCR amplification utilizing primer pairs derived from the 5′-UTR reported herein amplified a DNA fragment of the expected size from human genomic DNA whereas 5′-UTR primers derived from the Fukui et al. sequence did not yield a PCR product. The 5′-UTR of H1HR contains potential TATA and CCAAT boxes, a CACCC sequence, potential GREs and other DNA-binding motifs.