AMINO ACIDS DETERMINATION IN CHEESE

Abstract

Isotopic dilution gas chromatography-mass spectrometry (ID-GC/MS) is the techniques used for quantitative analysis of compounds labeled with stable isotopes. The aim of the work was to develop a simple and accurate analytical method to determine amino acids in some biological specimens by ID-GC/MS technique. A Trace DSQ ThermoFinnigan quadruple mass spectrometer coupled with a Trace GC was used. Amino acids were separated on a Rtx-5MS capillary column, 30 m x 0.25 mm, 0.25µm film thickness, using a temperature program from 50°C, 1 min, 6°C/min la 100°C, 4°C/min la 200°C, 20°C/min la 300°C, (3min). The free amino acids were determined during the ripening period.