[Study of changes in the parametres of oxidative stress, anti-oxidative defence and endothelial dysfunction in acute ischemia-reperfusion of the lower extremities in humans].

Abstract

We carried out a study on elderly people (70 +/- 2,2 years old) operated on as an acute occlusion of the femoral artery at its different anatomical levels. In those people, development of oxidative stress has been shown in injured lower extremity in the process of restoring of circulation. Marked increase in catalase activity in the blood gives evidence for work of the anti-oxidative defence system with tense. We determined an increase in aspartataminotransferase, alaninaminotransferase and creatininkinase in ischemia/reperfusion which are widely used to identify injures in muscular tissues. It has been shown that NO(2-) content in the blood at reperfusion depended on the level of nitrites and the duration ofischemia. Significant changes in NO metabolite content in the blood of the patients suffering from acute occlusion injures of the arteries in the lower extremities could be related with endothelial dysfunction.