Pharmacokinetics of human chorionic gonadotropin after i.m. administration in goats (Capra hircus)

Abstract

The present investigation addresses the pharmacokinetics of human chorionic gonadotropin (hCG), intramuscularly (i.m.) administered to goats. Nine pluriparous does of the Boer goat breed, 2-6 years of age and weighing 45-60 kg, were administered 500 IU hCG (2 ml Chorulon) deep into the thigh musculature 18 h after superovulatory FSH treatment. Blood samples were drawn from the jugular vein at 2 h intervals for the first 24 h, at 6 h intervals until 42 h, and at 12 h intervals until 114 h after administration. After centrifugation, plasma hCG concentrations were determined by electrochemiluminescence immunoassay. Pharmacokinetical parameters were as follows: lag time, 0.4 (S.E.M. 0.1) h; absorption rate constant, 0.34 (S.E.M. 0.002) h; absorption half-life, 2.7 (S.E.M. 0.5) h; elimination rate constant, 0.02 (S.E.M. 0.002) h; biological half-life, 39.4 (S.E.M. 5.1) h; and apparent volume of distribution, 16.9 (S.E.M. 4.3) l. The plasma hCG profile was characterized by an absorption phase of 11.6 (S.E.M. 1.8) h and an elimination phase of 70.0 (S.E.M. 9.8) h, with considerable individual variation in bioavailability and pharmacokinetical parameters. Biological half-life was negatively correlated (P<0.05) with peak concentration (r=-0.76), absorption rate constant (r=-0.78), and elimination rate constant (r=-0.87). The results indicate that after rapid absorption, hCG remains in the circulation for an extended period. This has to be taken into account when assessing the stimulatory response to hCG treatment on an ovarian level. © 2012 Society for Reproduction and Fertility.