Assay Linearity and Spike-Recovery Assessment in Optimization protocol for the analysis of Serum Cytokines by Sandwich ELISA Platform

Abstract

Background: A sandwich Enzyme-linked immunosorbent assay (sELISA) can be used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as quality control checks in industries. To generate quality and reliable data using this technique, it is essential to optimise the conditions of carrying out sELISA. The optimisation protocol for sELISA includes but not limited to the determination of the right dilution of the sample type, the optimal concentration for the primary antibodies, the use of correct diluent to mention a few.