Callus Induction and Plant Regeneration from Leaf Explants of Pepino

Abstract

Tobacco (Nicotiana tabacum L.) is a mode plant in the research of tissue culture, plant regeneration and other related fields. Tobacco leaves were used as explants in this experiment. Large amount calli were formed After leaves cultured in basic MS medium with intermediate ratio of auxin to cytokinin, and the callus induction rate was up to 100%. Some differences were found between the treatments of lightness and darkness: the callus cultured in lightness represent more tight structure and regular shape with the color of kelly, while that in darkness show itself less compact and irregular with the color of straw yellow. Subsequently, shoot regeneration was observed with the low ratio of auxin to cytokinin, which suggests that light wasn't the necessary element since shoot formed both in darkness and lightness and the No. of shoots had no significant difference. At the same time, slight differences were found in surface color of callus and the state of shoots.