CYTOTOXICITY ACTIVITY OF CHRISTIA VESPERTILIONIS LEAVES EXTRACTS AGAINST HELA CELLS AND THE DETECTION OF COMPOUNDS BY GC-MS

Abstract

Plants especially herbs have been utilized by old society for some therapeutic effects prior. In the present day, the notoriety of utilizing plants particularly for anticancer activity has continued expanding. Numerous examinations have been directed to separate out all phytochemical compounds contained in the herb that may be responsible of remedial impacts, for example, anticancer activity. In this research, Christia vespertilionis leaves extracts have been utilized to determine the cytotoxic activities of the plant extract towards human cervical cancer cell line (HeLa). C. vespertilionis leaves have been fractioned utilizing partitioned extraction from crude methanol extract followed by partitioning extract with increasing polarity namely hexane, dichloromethane (DCM), chloroform, n-butanol and aqueous fractions. Based on the discoveries, DCM extract of C. vespertilionis leaves has demonstrated the most elevated cytotoxicity activities towards HeLa cell line shown significantly by lowest half inhibitory concentration (IC50). DCM extract displayed highest cytotoxicity with IC50 estimation of 63.68, 55.42 and 53.04 µg/mL at 24, 48 and 72 hours respectively. Extensive cell detachment and the presence of apoptotic bodies in 24 hours may propose cytotoxicity activity of the extract. Additionally, the presence of various phytochemical compounds in the extract utilizing gas chromatography-mass spectrometry investigation, for example, 2-propenoic acid, hexadecanoic acid, cyclododecane,9,12,15-octadecatrienoic acid, and phytol may be the explanation behind the cytotoxic activities displayed by the extract onto the HeLa cell lines. It was recommended that this plant may have pharmacological significance in the future.