Development and characterization of microsatellite loci in the pantropical fern Hypolepis punctata (Dennstaedtiaceae)

Abstract

© 2015 Shang et al. Published by the Botanical Society of America. Premise of the study: Microsatellite loci were isolated in Hypolepis punctata (Dennstaedtiaceae) to further study the reproductive ecology of this species. Methods and Results: We developed 16 microsatellite loci from one sample of H. punctata using an enriched genomic library. These loci were characterized in 28 individuals. The number of alleles per locus ranged from two to 10, and the expected heterozygosity ranged from 0.036 to 0.845. Conclusions: The results indicate that the microsatellite markers can facilitate further studies on inferring the phylogeography and population genetics of H. punctata and related species.