Kinetic spectrophotometric determination of ascorbic acid in pharmaceutical samples by oxidation of ponceau 4R by hydrogen peroxide

Abstract

A new sensitive and simple kinetic method is developed for determination of traces of ascorbic acid based on its activated effect on oxidation of trisodium-2-hydroxy-1-(4-sulphonato-1-naphthylazo)naphthalene-6,8-disulphonato (red artificial color Ponceau 4R) by hydrogen peroxide, in the presence of Cu(II) as catalyst, in borate buffer. The reaction is followed spectrophotometrically by tracing the oxidation product at 478.4 nmwithin 1 min after addition of H2O2. The optimum reaction conditions are: borate buffer (pH = 11.00), Ponceau 4R (9.6·10-6 mol/L), H2O2 (2·10-2 mol/L), Cu(II) (8·10-7 mol/L) at 22°C. Following this procedure, ascorbic acid can be determined with a linear calibration graph up to 1.76 ng/mL and a detection limit of 0.28, based on 3S criterion. The relative error ranges between 6.77-1.66% for the concentration interval of ascorbic acid 1.76-17.61 ng/mL. The effects of certain foreign ions upon the reaction rate were determined for an assessment of the selectivity of the method. The method was applied for determination of ascorbic acid in pharmaceutical samples, and spectrophotometric method was used like an comparative method.