A green approach to the synthesis of novel "Desert rose stone"-like nanobiocatalytic system with excellent enzyme activity and stability

Abstract

3D hierarchical layer double hydroxides (LDHs) have attracted extensive interest due to their unique electronic and catalytic properties. Unfortunately, the existing preparation methods require high temperature or toxic organic compounds, which limits the applications of the 3D hierarchical LDHs in biocatalysis and biomedicine. Herein, we present a green strategy to synthesize "Desert Rose Stone"-like Mg-Al-CO3 LDH nanoflowersin situdeposited on aluminum substratesviaa coprecipitation method using atmospheric carbon dioxide. Using this method, we construct a novel "Desert Rose Stone"-like nanobiocatalytic system by using HRP as the model enzyme. Compared with the free HRP, the HRP/Mg-Al-LDH nanobiocatalytic system exhibits higher catalytic activity and stability. A smaller apparent Michaelis-Menten constant (0.16 mM) of this system suggests that the encapsulated HRP shows higher affinity towards H2O2.