A Study on the Intestinal Lipase of Indian Major Carp Labeo rohita

Abstract

Lipase from rohu(Labeo rohita, cyprinidae) intestine was extracted, fractionated with ammonium sulphate and purified on Sephadex G-100 column. It was found that the specific activity increased 3.6 times after ammonium sulphate precipitation and Sephadex G-100 Chro- matography of crude extract. Lipase activity at different levels of ammonium sulphate fraction- ations was tested. Precipitates obtained at 30 and 60% saturation of ammonium sulphate showed lower activity. Fractions precipitated with 80% saturated ammonium sulphate had the maximum activity and no activity was detected in the filtrate from this fraction. Lipase was found to have optimum activity at temperature 45°C and at pH 7.0. The amino acid composi- tion of the enzyme showed that lipase had high amount of polar amino acids than nonpolar amino acid content.