Influence of Dinitrosyl Iron Complexes on the Activity of Enzymes, Indicators of Cardiovascular Diseases

Abstract

Increased enzyme activities of matrix metalloproteinase, myeloperoxidase, renin, histone deacetylase, and poly (ADP-ribose) polymerase are indicators of the development of cardiovascular diseases. Therefore, the search for new inhibitors of these enzymes is relevant. The effect of iron dinitrosyl complexes and nitric oxide donors on these enzymes' activity was studied. It has been shown by fluorescence spectroscopy that DNICs (at concentration 2x10-4M) inhibit the activity of these enzymes. The constants of half-maximal inhibition of enzymes under the action of DNICs were determined (10-4-10-5M). The results showed that DNICs are effective inhibitors of matrix metalloproteinase, myeloperoxidase, renin, and polyadenine-ribose polymerase but do not affect histone deacetylase activity. These DNICs have been shown to have therapeutic potential for treating cardiovascular diseases.