Hematological impact in mice due to alteration in their nutritional style

Abstract

It is well established that alteration of diet style from plant-based to fat-rich diet lead to many complications including cardiovascular disease, metabolic syndrome, osteoporosis, and as well as anemia. According to our previous researches, changing in nutritional style from a conventional diet to fat-rich diet caused disturbance in the histology and function of different organs. So this work aims to study the effect of transition from a completely plant-based diet to 10% animal products on different hematology parameters in mice by using two groups of adult male Balb/c mice (n=8 mice for each). The first group represents restricted group that fed on conventional diet containing 10% sheep brain homogenate, while the second group represents control group that fed ad libitum on conventional diet only. The mice were sacrificed by cervical dislocation after 7 days. Blood samples were collected from the eyes in sterile tubes and immediately used to determine complete blood count (CBC). The results showed that platelets count, platelets/Lymphocytes ratio, and the total count of white blood cells particularly monocytes in restricted group are significantly higher than those in control group. However, Red blood cells RBCs, hemoglobin concentration, hematocrit, RDW-SD level are significantly lower in restricted group in comparison with those of control group. In conclusion, these results indicate that nutritional style alteration can affect cellular elements of blood and may provoke anemia, thromboembolism and immune response which need further investigation.