Effectiveness of coating acrylic resin dentures on preventing candida adhesion

Abstract

Purpose: The aim of this study was to prevent the adhesion of C. albicans on acrylic resin dentures by modifying their surfaces. Materials and Methods: Ninety acrylic resin plates were divided into three groups. Group I: conventionally processed acrylic resin plates. Group II: plates painted with 2-Octyl Cyanoacrylate adhesive. Group III: plates painted with Adper Single Bond Adhesive. All specimens were immersed separately in containers filled with artificial saliva that contained C. albicans and then incubated for 11 days at 37°C. Three methods of evaluation were used to count the adhered Candida: direct culture, slide count, and serial dilutions. Results: C. albicans in 1/10, 1/102, and 1/103 dilutions showed overgrowth in group I, while overgrowth was noted only with 1/10 dilution in group III. For group III, mean colony numbers of 123, 22, 3.4, and 0 were found for the 1/102, 1/103, 1/104, and 1/105 dilutions, respectively. Regarding the slide counts, group I showed a mean fungal count of 166 compared to 40 for group III with 1/10 dilution, 21 compared to 9 with 1/102 dilution, 8.6 compared to 0.7 with 1/103 dilution, and 1.2 compared to 0 with 1/104 dilution. No plates in group II showed any candidal colonies regardless of the method of evaluation (0%). These differences were statistically significant (p < 0.0001). Conclusion: Coating the acrylic resin dentures with Adper Single Bond Adhesive was effective in reducing C. albicans adhesion to dentures, while coating with 2-Octyl Cyanoacrylate adhesive completely inhibited such adhesion. © 2013 by the American College of Prosthodontists.