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A PCR-high-resolution melt assay for rapid differentiation of nontypeable Haemophilus influenzae and Haemophilus haemolyticus

Journal of Clinical Microbiology (2014) 52(2) 663-667
DOI: 10.1128/JCM.02191-13
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Abstract

We have developed a PCR-high-resolution melt (PCR-HRM) assay to discriminate nontypeable Haemophilus influenzae (NTHi) colonies from Haemophilus haemolyticus. This method is rapid and robust, with 96% sensitivity and 92% specificity compared to the hpd#3 assay. PCR-HRM is ideal for high-throughput screening for NTHi surveillance and clinical trials. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

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Pickering, J., Binks, M. J., Beissbarth, J., Hare, K. M., Kirkham, L. A. S., & Smith-Vaughan, H. (2014). A PCR-high-resolution melt assay for rapid differentiation of nontypeable Haemophilus influenzae and Haemophilus haemolyticus. Journal of Clinical Microbiology, 52(2), 663–667. https://doi.org/10.1128/JCM.02191-13

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