Simple, inexpensive, and rapid way to produce Bacillus subtilis spores for the Guthrie bioassay

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Abstract

Esculin agar has been found to be a simple, inexpensive, rapid, and reliable means to promote production of spores of inhibitor-sensitive clones of Bacillus subtilis strains ATCC 6051 and 6633 for use in the Guthrie bioassay screening tests for genetic metabolic disorders.

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Frankin, M. L., & Clark, W. A. (1981). Simple, inexpensive, and rapid way to produce Bacillus subtilis spores for the Guthrie bioassay. Journal of Clinical Microbiology, 14(1), 113–115. https://doi.org/10.1128/jcm.14.1.113-115.1981

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