Isolation and Characterization of Collagenase from Bacillus thuringiensis for Degrading Fish Skin Collagen of Cirata Reservoir Waste

Abstract

The objective of this research was to isolate and characterize collagenase of Bacillus thuringiensis obtained from the collection of Aquatic Biotechnology Laboratory, Faculty of Fisheries and Marine Science, Padjadjaran University.  The substrate of collagen was derived from skin Tilapia waste of Cirata Reservoar. This study showed the presence of clear zone which is a sign of colagenolitik activity of B. thuringiensis. The optimum production time of collagenase was 24 hours of incubation. Collagenase of crude extract had collagenase activity of 0.181 units/ml with the protein concentration was 0.640 mg/ml. It was also found that the optimum temperature of collagenase derived from crude extract was 50° C and the optimum pH was 7-9. Keywords: collagenase; Bacillus thuringiensis;  skin, waste