8- Hydroxy-Deoxyguanosine (8-OhDG) urine as a biomarker of oxidative damage in late elderly diabetes mellitus

Abstract

Diabetes Mellitus (DM) is a chronic metabolic disease characterized by high blood sugar levels that exceed normal limits. The DM pathomechanism involves oxidative stress, which has the potential to cause damage to cell components, including Deoxyribonucleic Acid (DNA). 8-hydroxy-deoxyguanosine (8-OhDG) is the most frequently detected oxidative DNA lesion. It is water-soluble and excreted in the urine. This study aimed to compare urine 8-OhDG levels in patients with controlled and uncontrolled blood sugar diabetes and healthy controls aged 56-65 years. Analytical descriptive research with a cross-sectional study design involved 80 participants determined with purposive sampling. The levels of 8-OhDG were measured using the Enzyme-Linked Immunosorbent Assay (ELISA) method. The results showed that urine 8-OhDG levels in DM patients (1.426±0.204ng/mL) were higher than in healthy controls (1.372±0.190ng/mL), although not statistically significant. Urine levels of 8-OhDG in DM patients aged 61-65 years (1.468±0.220ng/mL) were higher than in DM patients aged 56-60 years (1.394±0.190ng/mL), although not statistically significant. Urine levels of 8-Ohdg in controlled DM patient were lower (1.282±0.131 ng/mL) than in uncontrolled DM patient (1.569±0.159 ng/mL), were significantly different (p<0.05). 8-OhDG produced by the body is the final product of oxidative DNA damage.