Isolation, characterization, molecular identification of probiotic bacteria from meconium

Abstract

Probiotic bacteria are a group of bacteria that have positive effects such as increasing the absorption ability of some nutrients, maintaining the intestinal pH so that it can be protected from pathogenic microorganisms, maintaining disturbances in water absorption, smoothing digestion by producing several digestive enzymes. Each bacterial species has different probiotic effects so selection and identification are needed to get a good strain of probiotics. The identification of probiotic bacteria in this study uses the molecular identification method with the 16S rRNA marker gene. 6 probiotic bacterial isolates (DAI, DA3, DA4, DA7, DA8, DA10) were identified as having the characteristics of a circular shape, the edge of the entire and flat elevation, negative. The antibacterial test results have a clear zone of 7.1 mm. The results of sequencing with the help of the Basic Local Alignment Search Tool (BLAST) program showed that probiotic bacteria from meconium had 99.78% similarity with Basillus coagulans NBRC.