Sodium nitroprusside and indole acetic acid improve the tolerance of tomato plants to heat stress by protecting against DNA damage

Abstract

Climate change represents a major threat to agriculture. High ambient temperatures, as a result of global warming, are currently limiting plant growth and development. The aim of the present study was to investigate the effect of sodium nitroprusside (SNP) in combination with indole acetic acid (IAA) on tomato (Lycopersicon esculentum Mill.) plants under heat stress (HS) and non-heat stress (non-HS) conditions. HS is suggested to induce the formation of reactive oxygen species, such as superoxide and hydrogen peroxide, which may lead to genotoxicity by damaging DNA, which can be detected by the comet assay (single-cell gel electrophoresis). HS substantially enhanced proline (Pro), malondialdehyde accumulation, electrolyte leakage (EL), growth reduction, and reduced physiological and biochemical parameters. However, the co-application of SNP and IAA alleviated the adverse effects of HS by promoting catalase, peroxidase, and superoxide dismutase activities and enhancing the accumulation of photosynthetic pigments (chlorophyll a and b) and Pro with a concomitant decrease in H2O2 and O2 content, EL, and DNA damage. Conversely, the treatment of tomato plants with the NO scavenger cPTIO [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide] along with SNP and IAA further reduced the SNP signal. Therefore, these results suggest that the application of SNP with IAA improves plant defense mechanisms against HS.