Evaluation of in vitro inhibition of proton-pump by nanosilver particles synthesized using seeds of anethum graveolens

Abstract

Objective: The green synthesis of nanoparticles is gaining popularity owing to its several advantages over the chemical methods. This study reports the biosynthesis of nanosilver mediated by the extract of the seeds of Anethum graveolens. Methods: Green synthesis of silver nanoparticles (AgNPs) was carried out using the aqueous extract of the seeds of A. graveolens (AESE). Microwave irradiation was done to hasten the process. The AgNPs synthesized were physically characterized using ultraviolet-visible spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy. The potential of the nanosilver particles in inhibiting H+ K+ adenosine triphosphatase (proton pump) was evaluated. Results: The nanosilver particles were initially subjected to characterization studies to ascertain their shape, size and surface composition. The particles were found to be crystalline, spherical of size 30-35 nm. The nanosilver particles inhibited the activity of proton pump by 67 % and thus were as potential as the standard drug, omeprazole. Conclusion: The seeds have several pharmacological benefits including its gastroprotective potential and hence the nanosilver particles synthesized using AESE was assessed for its gastroprotective potential. The results of this study prove that the nanosilver synthesized by green technology can inhibit proton-pump and thus act as promising gastroprotective agents.