Studies on Phytochemistry and Wound Healing Activity ofLeaf Extract Ointment of Clerodendrum infortunatum inCanines

Abstract

The present study was carried out to evaluate the efficacy of ointments containing leaf extract of Clerodendrum infortunatum for treating wounds in canines in field cases. The 50% ethanolic and methanolic extract of the plant was subjected to preliminary phytochemistry and GC-MS analysis to assess the presence of active phytoconstituents. The wound healing was assessed with percent wound contraction on 0, 4th, 8th, 12th, 16th, and 20th day and epithelization period in 30 canines presented with a history of wound at VCC, private clinics, and dog shelters run by NGOs. The 10% w/w ointment of both the extract was used and it was compared with commercially available Povidone Iodine ointment USP 5% (Betadine®). The preliminary phytochemical analysis of the extracts showed the presence of glycosides, alkaloids, carbohydrates, amino acids, phytosterol, phenolic compounds, and flavonoids. Whereas, GC-MS analysis revealed the presence of 21 compounds in ethanolic extract and 24 compounds in methanolic extract. The wound contraction in the methanolic extract ointment treated group was most significant and also demonstrated an epithelization period of 17.5 ± 1.210 days as compared to 22.8 ± 0.525 and 20.9 ± 0.669 in Povidone Iodine ointment and ethanolic extract ointment treated groups, respectively. The finding of the study suggests that the ointment made from the extract of Clerodenrum infortunatum leaves possesses promising wound healing activity.