Effect of Mn2+Addition on Delignification of Water Hyacinth using Phanerochaete Chrysosporium

Abstract

In order to prepare water hyacinth as a bioethanol feedstock, it is necessary to perform a pretreatment process. Biological process is considered to be one of the potential pretreatment that may be developed in the future. This study aims to increase lignin degradation rate and shorten the pretreatment time and increase fermentable sugar yield after enzymatic hydrolysis. Experiment was conducted in batch mode. Cut water hyacinth prepared was inoculated with the fungus and incubated at room temperature for 28 days during which its content of lignin, cellulose and hemicellulose was analyzed regularly. Effect of co-factor was studied by adding Mn2+ with varied concentrations (between 0.05 – 1%) to water hyacinth The results showed increase the rate of degradation of lignin and increase fermentable sugar yield results with the addition of co-factor Mn2+. In Pretreatment of Water Hyacinth without addition of Mn2+ occured lignin degradation reached 24.39% during 16 days incubated with the fungus and fermentable sugar yield reached 36.37%. An addition of co-factor Mn2+ at a concentration of 0.5% showed that the lignin degradation reached 42.44% during 16 days of incubation, and the fermentable sugar yield in hydrolysis increased to reach 67.66%.