17β-hydroxysteroid dehydrogenase type 11 is a major peroxisome proliferator-activated receptor α-regulated gene in mouse intestine

Abstract

In order to study the role of peroxisome proliferator-activated receptor α in mouse intestine, its agonist-induced proteins were identified by peptide mass fingerprinting followed by Northern blot analysis using their cDNAs. One of the most remarkably induced proteins was identified as 17β-hydroxysterol dehydrogenase type 11. Its very rapid induction by various agonists was most efficient in intestine and then in liver. These findings together with recently reported results showing the enzyme family's wide substrate spectrum, including not only glucocorticoids and sex steroids but also bile acids, fatty acids and branched chain amino acids, suggest new roles for both peroxisome proliferator-activated receptor α and 17β-hydroxysterol dehydrogenase type 11 in lipid metabolism and/or detoxification in the intestine.