Mitochondrion is the main intracellular site for thermogenesis and attractive energy expenditure targeting for obesity therapy. Here, we develop a method of mitochondrial thermometry based on Rhodamine B methyl ester, which equilibrates as a thermosensitive mixture of nonfluorescent and fluorescent resonance forms. Using this approach, we are able to demonstrate that the efficacy of norepinephrine-induced thermogenesis is low, and measure the maximum transient rate of temperature increase in brown adipocytes. Electronic Supplementary Material The online version of this article (doi:10.1007/s41048-017-0039-6) contains supplementary material, which is available to authorized users.
CITATION STYLE
Xie, T.-R., Liu, C.-F., & Kang, J.-S. (2017). Dye-based mito-thermometry and its application in thermogenesis of brown adipocytes. Biophysics Reports, 3(4–6), 85–91. https://doi.org/10.1007/s41048-017-0039-6
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