Anti-inflammatory and cytotoxic activities of constituents from Wedelia trilobata (L.) Hitchc.

Abstract

Using various chromatographic separations, five compounds (1–5) were isolated from a methanol extract of W. trilobata leaves. Their structures were elucidated to be paniculoside-IV (1), 3-O-[β-D-glucopyranosyl(1-4)-β-D-glucoronopyranosyl] oleanolic acid 28-O-β-D-glucopyranosyl ester (2), 5,7,4'-trihydroxyflavone (3), apigenin 7-O-β-D-glucopyranoside (4), and 3,4-dihydroxy-cinnamic acid (5), by detailed analysis via spectroscopic techniques (IR, 1D, 2D NMR and ESI-MS data) as well as comparison with those reported. This is the first report of 2 from Wedelia genus. Furthermore, the effects of the isolated compounds on LPS-induced NO production in RAW264.7 cells and cytotoxic activity (against A549, MCF-7, Hep3B, and PC3 cell lines) were also evaluated.