Effect of different molecular weight on the antioxidant activity and physicochemical properties of golden apple snail (Ampullariidae) protein hydrolysates

Abstract

Golden apple snail (GAS) is reported to have a medicine effect for treat broken bones, back pain and stool bloody. This study was aimed to investigate the fractionation of protein hydrolysate from the golden apple snail (GAS) using an ultrafiltration technique with molecular weight cut-offs of 3, 5, and 10 kDa, followed by the determination of its antioxidant activity and functional properties. In terms of antioxidant activity, fraction of 3 kDa showed the highest reducing power and DPPH radical scavenging activity. Additionally, fraction of 5 kDa had the highest chelating effect on ferrous ion (85.6%). The results also revealed that fraction of 3 kDa showed good emulsion activity index and water binding capacity, while 5 kDa showed good foaming capacity and solubility. These results suggested that GASH showed potential for application in the food industry as its antioxidant, with strong functional properties able to be obtained by varying its molecular weights.