Screening by NMR: A new approach for the study of bioactive natural products? The example of Pleurotus ostreatus hot water extract

Abstract

Direct NMR screening of natural products obtained from hot water extracts of medicinal species is accomplished through STD and tr-NOESY experiments on a crude mixture and a given protein receptor. It is shown, with use of a mushroom extract as model case, that this protocol may provide a fast and simple method, particularly useful in natural products chemistry, through which to detect the presence of ligands for a target receptor. © Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2005.