A golgi-localized mannosidase (man1b1) plays a nonenzymatic gatekeeper role in protein biosynthetic quality control

Abstract

Background: The capacity of Golgi-localized MAN1B1 to promote ERAD was investigated. Results: The MAN1B1 catalytic domain is dispensable for ERAD, which helped identify an important conserved, non-enzymatic decapeptide sequence within the luminal stem. Conclusion: The tempo-spatial expansion of quality control in the secretory pathway includes evolutionary gain-of-function for MAN1B1. Significance: Evolutionary distinctions can exist in how quality control systems operate. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.