Separation and purification of polyphenols from Pericarpium Granati using macroporous resins and evaluation of its anti-Streptococcus mutans activity in vitro

Abstract

Background: The optimal parameters of separation and purification of polyphenols from Pericarpium Granati were determined and the antibacterial activities against S. mutans in vitro were investigated. Methodology: Through comparing the static adsorption/desorption properties of five kinds of tested macroporous resins for polyphenols, HPD-450 resin was selected to investigate optimal purification parameters by dynamic curve experiments. The optimal purification conditions as follows: 9.0 BV of feeding solution at concentration of 3.2 mg mL-1 through the column at 3.0 BV h-1, then for desorption 60% ethanol-aqueous 3.5 BV at 2.0 BV h-1 eluted. The content of polyphenols was increased from 34.9±1.6% to 71.1±3.1% with a recovery yield of 85.5±3.7% according to the optimal parameters. Results: The present study showed that the MIC of purity of polyphenols was 6.25 mg mL-1. The acidogenicity of S. mutans can be significantly inhibited when the concentration was up to or higher than 1.56 mg mL-1 and the adherence of S. mutans can be significantly inhibited when the concentration was up to or higher than 3.13 mg mL-1 as well. Conclusion: The results demonstrated that polyphenols from Pericarpium Granati have significant activity against S. mutans and it is a good candidate for further development as an oral care agent for preventing dental caries.