Sensitive in situ hybridisation technique, using biotin-streptavidin-polyalkaline phosphatase complex

Abstract

A sensitive in situ hybridisation technique, using a biotin-straptavidin-polyalkaline phosphatase complex detection system, was successfully applied to smears of fresh cultured cells, frozen sections, and formalin fixed paraffin processed tissue: the procedure was successful for DNA- DNA hybridisations using a variety of DNA probes. The detection method is rapid, reliable, and economical producing a purplish-blue precipitate at the site of hybridisation and clearly visible by low power light microscopy.