A new method for counting of quail leukocytes by flow cytometry

Abstract

An automatic counting method was developed for fish blood cells using a fluorescent dye, 3, 3-dihexyloxacarbocyanine (DiOC6(3)), that selectively stain lipid bilayers in living cells. In the present study, the DiOC6(3) method was applied to quail (Cotumix cotumix japonica) blood cells. After quail blood cells were stained with DiOC6(3), absolute counts and relative proportions of erythrocytes, granulocytes, monocytes, and lymphocytes plus thrombocytes in whole blood were obtained by means of flow cytometry (FC). The number of each cell types by the FC was in good agreement with those counted microscopically. This method will offer new possibilities for routine blood cell counting for avian medicine.